Cellular & Molecular Biology Lab provides techniques, equipments and protocols to investigate musculoskeletal disorders at cellular and molecular levels.
The laboratory can realize recognizing, analyzing and manipulating cells as well as biomolecular with efficiency, sensitiveness and high throughput. So far, the lab has established the following techniques:
Ø Cell Techniques
It includes cell separation, cell culture, cell fusion, cell activity analysis, cell immunophenotyping analysis, cell circle analysis and so on.
Ø Nucleic Acid Techniques
It includes DNA / RNA extraction, gene splicing, gene transduction, gene transfection, gene clone, gene expression, nucleic acid aptamer screening and so on.
Ø Protein Macromolecular Techniques
It includes protein extraction, protein purification, protein separation, protein quantitative / qualitative analysis, protein-protein interaction analysis, nucleic acid aptamer-protein analysis interaction and so on.
Ø Equipment Name: Biological Safety Cabinets
Ø Location: Rm. 608, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: ThermoScientific* Herasafe KS biological safety cabinets deliver maximum safety, comfort and
convenience. Featuring an advanced design, these cabinets are ideal in research, pharmaceutical and clinical facilities.
Ø Equipment Name: CO2 Incubator
Ø Location: Rm. 608, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: Thermo Scientific* Heracell i series tri-gas incubators allow convenient oxygen control and are available in
noncorrosive stainless steel or 100% pure copper. Thermo Scientific Heracell itri-gas incubators provide the ideal in vitro
environment: clean, reliable and easy to use, protecting valuable samples while optimizing cell growth.
Ø Equipment Name: Ultracentrifuge
Ø Location: Rm. 609, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: The Beckman Coulter Optima TLX Ultracentrifuge is a table top ultracentrifuge that can be used for a
variety of high speed applications. This system has an easy to use control panel a temperature control system a
self-purging vacuum system a programmable memory that can store up to 10 different profiles and more.
Because this high speed system has a maximum speed of 120000rpm, it comes with safety features that include
a steel chamber door which has an electromechanical door locking mechanism.
Ø Equipment Name: Flow Cytometer
Ø Location: Rm. 601, SCM
Ø Technician: Ms. Sally Lee (sallylee@hkbu.edu.hk)
Ø Description: Employed in cell counting, sorting, biomarker detection and protein engineering, by suspending cells in a
stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis
of the physical and / or chemical characteristics of up to thousands of particles per second.
Ø Equipment Name: Real-Time PCR Instrument
Ø Location: Rm. 609, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: Provide a technique based on the polymerase chain reaction, which is used to amplify and
simultaneously quantify a targeted DNA molecule. For one or more specific sequences in a DNA sample,
Real Time-PCR instrument enables both detection and quantification.
Ø Equipment Name: 2D Gel Electrophoresis System
Ø Location: Rm. 609, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: Bio-Rad two dimensional (2D) gel electrophoresis system is considered to be the best option for high-
resolution profiling of low abundance proteins. The analysis of complex protein samples can be tedious, time-
consuming and expensive. Recent advancements in sample fractionation and 2D electrophoresis enable researchers
to overc ome these problems in identifying low abundance proteins in complex biological samples.
Ø Name: Molecular Imager System
Ø Location: Rm. 609, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: The Molecular Imager® Gel Doc™ XR documentation system is a fast, easy-to-use and high-resolution
gel imaging system. It includes darkroom; camera; UV- and white-light illumination; filter slider with amber filter; and
UV-protection shield. Easy-to-use "capture and print" Quantity One® 1-D analysis software and unlimited copies of
Quantity One Basic software are also included.
Ø Equipment Name: UV Spectrophotometer
Ø Location: Rm. 609, SCM
Ø Technician: Ms. Hilda Cheung (hildacys@hkbu.edu.hk)
Ø Description: The T70 UV Spectrophotometer is a dual beam instrument with a fixed 2nm band pass. The wavelength
range is 190 - 1100nm. The T70 has a large 'fold away' LCD display unit which allows to complete display of Spectra.
The system has a large easy to use positive touch key pad and the instrument can be used a complete standal one
unit.
Protocol for Cell-SELEX
